1/5 Soil+Seawater Medium

For some marine and brackish-water algae requiring salinities at 6 ppt, glass-distilled water and pasteurized, filtered seawater are combined prior to steaming, such that 1/5 Soil+Seawater contains seawater combined with distilled water 1:4.

Directions

For 200 mL Total

1. Combine all components.
2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
3. Refrigerate (10 °C) 24 hours or more and bring to room temperature before using.