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Modified Desmidiacean MediumModified Desmidiacean Medium The final medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber lined screw caps. Directions For 1 L Total To approximately 900 mL of dH2O add the first 7 components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. Cover and autoclave medium. Allow to cool. Add 1 sterile barley grain per test tube then store at refrigerator temperature. #
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Modified Desmidiacean Medium
The final medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.
Directions
For 1 L Total
- To approximately 900 mL of dH2O add the first 7 components in the order specified while stirring continuously.
- Bring total volume to 1 L with dH2O.
- Cover and autoclave medium.
- Allow to cool.
- Add 1 sterile barley grain per test tube then store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | KNO3 (Baker 3190) |
10 mL/L | 1 g/100 mL | 0.9 mM |
| 2 | (NH4)2HPO4 (Fisher A686) |
5 mL/L | 0.2 g/100 mL | 0.075 mM |
| 3 | MgSO4•7H2O (Sigma 230391) |
10 mL/L | 0.1 g/100 mL | 3.3 µM |
| 4 | CaSO4•2H2O saturated solution (Mallinckroft 4300) |
10 mL/L | ||
| 5 | Soilwater: GR+ Medium | 20 mL/L | ||
| 6 | Soilwater: Peat Medium | 10 mL/L | ||
| 7 | MWC Metal Solution | 5 mL/L | ||
| 8 | Barley grains autoclaved | 1 grain/10 mL |
Modified Desmidiacean Medium
Item no
:
20057526922
US$ 60.00
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Min. order:
1piece
Shipping Estimate
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Ships within 48 hours · Estimated delivery Jun 21 - Jun 26
US$40
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Exchange/Return Notes
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