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Desmid MediumDesmid Medium Modification of Pringsheim's desmidiacean medium. Directions For 1 L Total To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. For 1. 5% agar medium pour 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Soilwater: GR+
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Desmid Medium

Modification of Pringsheim's desmidiacean medium.

Directions

For 1 L Total
  1. To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
    • For 1.5% agar medium pour 15 g of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Soilwater: GR+ Medium 50 mL/L
2 MgSO47H2O
(Sigma 230391)
10 mL/L 0.2 g/200 mL dH2O 0.04 mM
3 K2HPO4
(Sigma P 3786)
10 mL/L 0.2 g/200 mL dH2O 0.057 mM
4 KNO3
(Baker 3190)
10 mL/L 2 g/200 mL dH2O 0.99 mM

Desmid Medium

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