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DYIII MediumDYIII Medium Modification of Sandgren's recipe. Suitable for xenic cultures of freshwater chrysophytes. Directions For 1 L Total To approximately 950 mL of dH2O add each of the components in the order specified (except vitamins) while stirring continuously. Adjust the pH to 6. 7. Bring the total volume to 1 L with dH2O. For 1. 5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add vitamins. For agar
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DYIII Medium
Modification of Sandgren's recipe. Suitable for xenic cultures of freshwater chrysophytes.
Directions
For 1 L Total
- To approximately 950 mL of dH2O add each of the components in the order specified (except vitamins) while stirring continuously.
- Adjust the pH to 6.7.
- Bring the total volume to 1 L with dH2O.
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- When cooled add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | MES (CAS: 7365-45-9) |
1.95 g/L | 10 mM | |
| 2 | MWC Metal Solution | 10 mL/L | ||
| 3 | CaCl2•2H2O (CAS: 10035-04-8) |
1 mL/L | 2 g/100 mL | 0.14 mM |
| 4 | MgSO4•7H2O (CAS: 10034-99-8) |
1 mL/L | 7.4 g/100 mL | 0.3 mM |
| 5 | Na2Glycerophosphate.5H2O (CAS: 13408-09-8) |
1 mL/L | 1 g/100 mL | 0.03 mM |
| 6 | NaNO3 (CAS: 7631-99-4) |
1 mL/L | 2 g/100 mL | 0.24 mM |
| 7 | Na2SiO3•9H2O (CAS: 13517-24-3) |
1 mL/L | 1.5 g/100 mL | 0.05 mM |
| 8 | NH4NO3 (CAS: 6484-52-2) |
1 mL/L | 1 g/100 mL | 0.12 mM |
| 9 | KCl (CAS:7447-40-7) |
1 mL/L | 1 g/100 mL | 0.13 mM |
| 10 | Vitamin B12 | 1 mL/L | ||
| 11 | Biotin Vitamin Solution | 1 mL/L | ||
| 12 | Thiamine Vitamin Solution | 1 mL/L |
DYIII Medium
Item no
:
45747717197
US$ 55.00
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