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Snow Algae MediumSnow Algae Medium Suitable for snow algae collection. Directions For 1 L Total To approximately 500 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring the total volume to 1 L with dH2O, pH is 5. 1 5. 3. For 1. 5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1
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Snow Algae Medium
Suitable for snow algae collection.
Directions
For 1 L Total
- To approximately 500 mL of dH2O, add each of the components in the order specified while stirring continuously.
- Bring the total volume to 1 L with dH2O, pH is 5.1-5.3.
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | CaCl2•2H2O (Sigma C-3881) |
1 mL/L | 0.5 g/100 mL dH2O | 0.03 mM |
| 2 | NaNO3 (Fisher BP360-500) |
1 mL/L | 2.5 g/100 mL dH2O | 0.29 mM |
| 3 | NH4Cl (Fisher A 649-500) |
1 mL/L | 0.5 g/100 mL dH2O | 0.093 mM |
| 4 | CaSO4•2H2O (Mallinckroft 4300) |
1 mL/L | 0.4 g/100 mL dH2O | 0.023 mM |
| 5 | MgSO4•7H2O (Sigma 230391) |
1 mL/L | 0.5 g/100 mL dH2O | 0.02 mM |
| 6 | Na2SiO3•9H2O (Sigma 307815) |
1 mL/L | 0.2 g/100 mL dH2O | 0.007 mM |
| 7 | FE EDTA | 250 mL/L | ||
| 8 | Minor Nutrients | 10 mL/L | ||
| 9 | K2HPO4 (Sigma P 3786) |
2 mL | 3.48 g/100 mL dH2O | 0.4 mM |
| 10 | KH2PO4 (Sigma P 0662) |
98 mL | 2.72 g/100 mL dH2O | 0.02 M |
Snow Algae Medium
Item no
:
85586318179
US$ 55.00
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1piece
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