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P49 MediumP49 Medium Suitable for some volvocales, especially Chlamydomonas and Pandorina, which may benefit primarily from the ammonium component, modified volvox medium. Directions For 1 L Total To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously. Adjust the pH to 7. 0 Bring total volume to 1 L with dH2O. For 1. 5% agar medium add 15 grams of agar into the flask; do not mix. Cover
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P49 Medium
Suitable for some volvocales, especially Chlamydomonas and Pandorina, which may benefit primarily from the ammonium component, modified volvox medium.
Directions
For 1 L Total
- To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
- Adjust the pH to 7.0
- Bring total volume to 1 L with dH2O.
- For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
- Cover and autoclave medium.
- When cooled add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | MgSO4•7H2O (CAS: 10034-99-8) |
1 mL/L | 4 g/100 mL dH2O | 0.16 mM |
| 2 | Na2glycerophosphate•5H2O (CAS: 13408-09-8) |
1 mL/L | 5 g/100 mL dH2O | 0.16 mM |
| 3 | KCl (CAS: 7447-40-7) |
1 mL/L | 5 g/100 mL dH2O | 0.67 mM |
| 4 | Glycylglycine (CAS: 556-50-3) |
10 mL/L | 5 g/100 mL dH2O | 3.8 mM |
| 5 | P-IV Metal Solution | 6 mL/L | ||
| 6 | NH4NO3 (CAS: 6484-52-2) |
10 mL/L | 1 g/100 mL dH2O | 1.2 mM |
| 7 | CaCl2•2H2O (CAS: 10035-04-8) |
10 mL/L | 0.74 g/100 mL dH2O | 0.5 mM |
| 8 | Yeast extract (CAS: 8013-01-2) |
0.2 g/L | ||
| 9 | Tryptone (CAS: 91079-40-2) |
0.4 g/L | ||
| 10 | Vitamin B12 | 1 mL/L | ||
| 11 | Biotin Vitamin Solution | 1 mL/L | ||
| 12 | Thiamine Vitamin Solution | 1 mL/L |
P49 Medium
Item no
:
94559479196
US$ 55.00
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