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For marine algae that require salinities at 15 ppt, the basic Enriched Seawater Medium is combined with sterile distilled water prior to inoculating the new culture, such that 1/2 Enriched Seawater Medium combines Enriched Seawater Medium and sterile distilled water 1:1.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | Enriched Seawater Medium | 500 mL | ||
| 2 | sterile dH2O | 500 mL |
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