HLA-A Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-68347-60, E-AB-68347-120, E-AB-68347-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: HLA-A

Target Synonym: HLAA; HLA-A

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P04439,P30443

Background: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

Concentration: 1 mg/mL

Immunogen: Recombinant fusion protein of human HLA-A

Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:20

Calculated MW: 40kDa

Observed MW: 45KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only