Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active)

Size: 50μg

Catalogue Number: PKSH030690-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Alkaline Phosphatase; ALPI

Target Symptom: IAP

Research Area: Signal Transduction; Tags & Cell Markers; Cell biology; Cancer; immunology; metabolism;

Target Species: Human

Expression Host: HEK293 Cells

Application: Enzyme

Fusion Tag: C-His

UNIProt ID: P09923

Accession: P09923

Background: ALPI encodes for intestinal phosphatase alkaline; a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.

Activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 1000 pmoles/min/μg.

Sequence: Met 1-Asp 503

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 54 kDa

Observed MW: 66 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only