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Bristol MediumBristol Medium H. C. Bold's modification of Bristol's recipe (Bold 1949). General purpose freshwater medium and as bristol's solution, an essential component of other media see Bold 1NV, Bold 3N, Bristol NaCl, LDM, Proteose, Soil extract, and Trebouxia. Directions For 1 L Total To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. *For 1. 5% agar medium add
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Bristol Medium
H.C. Bold's modification of Bristol's recipe (Bold 1949). General purpose freshwater medium and as bristol's solution, an essential component of other media--see Bold 1NV, Bold 3N, Bristol-NaCl, LDM, Proteose, Soil extract, and Trebouxia.
Directions
For 1 L Total
- To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously.
- Bring total volume to 1 L with dH2O.
- *For 1.5% agar medium add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (Fisher BP360-500) | 10 mL/L | 10 g/400mL dH2O | 2.94 mM |
| 2 | CaCl2•2H2O (Sigma C-3881) | 10 mL/L | 1 g/400mL dH2O | 0.17 mM |
| 3 | MgSO4•7H2O (Sigma 230391) | 10 mL/L | 3 g/400mL dH2O | 0.3 mM |
| 4 | K2HPO4 (Sigma P 3786) | 10 mL/L | 3 g/400mL dH2O | 0.43 mM |
| 5 | KH2PO4 (Sigma P 0662) | 10 mL/L | 7 g/400mL dH2O | 1.29 mM |
| 6 | NaCl (Fisher S271-500) | 10 mL/L | 1 g/400mL dH2O | 0.43 mM |
Bristol Medium
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35679674294
US$ 55.00
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